Life in the Earth’s interior is as productive as in some ocean waters


Terrestrial and marine habitats have been considered the ecosystems with the highest primary production on Earth by far. Microscopic algae in the upper layers of the oceans and plants on land bind atmospheric carbon (CO2) and produce plant material driven by photosynthesis. Since sunlight does not penetrate into the subsurface, hardly any such primary production is to be expected.

However, genetic analyses of microorganisms in groundwater have indicated that even here many microorganisms are capable of primary production. In the absence of light, they must obtain the energy from oxidizing inorganic compounds, like from reduced sulfur of the surrounding rocks. However, the role of primary producers in the subsurface had never been confirmed before.

Groundwater is one of our most important sources of clean drinking water. The groundwater environment of the carbonate aquifers alone, which is the focus of the study, provides about ten percent of the world’s drinking water. With this in mind, the researchers carried out measurements of microbial microorganism carbon fixation in a subsurface aquifer, 5 to 90 meters belowground.

Surprisingly high primary production rates in total darkness

“The rates we measured were much higher than we anticipated,” says the first author of the study Dr. Will Overholt, Postdoctoral Researcher at Friedrich Schiller University Jena. “They equal carbon fixation rates measured in nutrient-poor marine surface waters and are up to six-fold greater than those observed in the lower zones of the sunlit open ocean, where there is just enough light for photosynthesis”.

Based on the measured carbon fixation rates, the researchers conservatively extrapolated global primary production in carbonate groundwater to be 110 million metric tons of carbon per year. Collectively, the net primary productivity of approximately 66 percent of the planet’s groundwater reservoirs would total 260 million metric tons of carbon per year, which is approximately 0.5 percent that of marine systems and 0.25 percent of global net primary production estimates.

“This may sound small but these measurements represent only our first estimate of what the true global value may be,” says senior author Prof Kirsten Küsel from the University of Jena and iDiv. “Since there is very little energy available in these nutrient-poor and permanently dark habitats, even a small percentage of the global primary production is a surprise.”

The researchers also sought to identify the microorganisms responsible for fixing carbon and generating new biomass within the aquifer. Metagenomic analyses point to a highly abundant microorganism not closely related to previously studied bacteria, within an uncharacterized order of Nitrospiria. “As food, these organisms are thought to form the basis of life for the entire groundwater ecosystem with all of its thousands of microbial species, similar to the role algae play in the oceans or plants on land,” says Overholt.

Unique method to measure primary production of microorganisms in aquifers

Measuring carbon fixation can be done with radioactively labeled carbon dioxide. “In carbonate rock environments, there is abundant dissolved CO2, that can make it difficult to directly observe rates of carbon fixation,” says Prof Susan Trumbore from the Max Planck Institute for Biogeochemistry in Jena. The team, therefore, used a special method to trace a small amount of labeled CO2 using highly sensitive accelerator mass spectrometry. “It is exciting to see what new insights these methods can lead to,” she says.

“Our findings offer new insights into how these subsurface ecosystems function, giving clues on how to monitor or remediate groundwater sources,” says Kirsten Küsel.

The research was published in Nature Geoscience.

More information: Kirsten Küsel, Carbon fixation rates in groundwater similar to those in oligotrophic marine systems, Nature Geoscience (2022). DOI: 10.1038/s41561-022-00968-5.
Journal information: Nature Geoscience.
Provided by German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig.

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